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Fig. 3.

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Fig. 3. Time kinetics of α-lipoic acid (α-LA)-induced apoptosis in A549 cells. (A) A549 cells were treated with 1 mM of α-LA for the designated time periods, and apoptosis was analyzed by determining the sub-G1 fraction of cells by fluorescence activated cell sorting (FACS). (B) Equal amounts of cell lysates were subjected to electrophoresis and expression of glucose regulated protein 78 (GRP78), C/EBP-homologous protein (CHOP), poly ADP-ribose polymerase (PARP), X-linked inhibitor of apoptosis protein (XIAP), and caspase 3 was evaluated by western blot analysis. Heat shock cognate protein 70 (Hsc 70) was used as a protein loading control.
Korean J Thorac Cardiovasc Surg 2012;45:1~10
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