The Korean Journal of Thoracic and Cardiovascular Surgery : eISSN 2093-6516 / pISSN 2233-601X

Fig. 4.

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Fig. 4. Induction of the endoplasmic reticulum (ER) stress-related response and X-box binding protein (XBP) splicing by α-lipoic acid (α-LA). (A) A549 cells treated with 1 mM α-LA or 100 µM cisplatin were stained with an ER tracking dye. (B) α-LA induced proteins related to ER stress. A549 cells were treated with various concentrations of α-LA for 18 hours. Equal amounts of cell lysates were subjected to electrophoresis and the expression of poly ADP-ribose polymerase (PARP), KDEL, C/EBP-homologous protein (CHOP), caspase 3, and myeloid cell leukemia 1 (Mcl-1) was determined by western blot analysis. Heat shock cognate protein 70 (Hsc 70) was used as a protein loading control. (C) α-LA induced X-box binding protein (XBP) mRNA splicing. mRNA from A549 cells treated with α-LA was isolated, and XBP-1 transcripts were detected by reverse transcription-polymerase chain reaction.
Korean J Thorac Cardiovasc Surg 2012;45:1~10
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